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Competitors such as Caster Semenya have endured intense public scrutiny, but also have in the end accomplished acceptance in their sport. Obligatory testing of all female competitors started in the 1960's and continued till 1999 (Figure 3). Initial tests in the 1960's had been primarily based on phenotypic traits by direct physical and/or gynecologic examination. Such tests would have potential to recognize as males each XX athletes with masculinization due to CAH, and XY competitors with incomplete AIS or partial genital masculinization due to 5a-reductase deficiency. At very first, chromosomal tests verified the presence of a Barr body in buccal smears (Simpson et al, 2000). By Barr physique screening, male competitors with Klinefelter's syndrome or female athletes with CAH are identified as female, but ladies with AIS are identified as male. Later tests screened female athletes for the presence of a Y chromosome, and subsequently for the SRY gene on the Y chromosome (Simpson et al, 2000). As with the Barr physique, testing for SRY identifies AIS ladies as male.